Histological evaluation of the osteoinductive potential of demineralized dentin matrix

  • Manoel Eduardo de Lima Machado University of São Paulo
  • Victor Di Donato Marques
  • Cleber Keiti Nabeshima University of São Paulo
Keywords: Biocompatible materials, dental implantation, dentin,


Objective: The aim of this study was to verify the osteoinductivity of the xenogeneic and ectopic implantation of demineralized dentin matrix (DDM) in muscle tissue of rats in short-term. Methods: Ten human teeth were utilized to obtain the DDM. Surgical access was made by incisions on two pockets created in the dorsal musculature of 10 Wistar rats, where the implants were inserted. The animals were divided into 2 groups: in the first group the implants remained in place for one month and in the second group for two months. Biopsies were taken at the end of the experimental times. Results: Histological examination showed the presence of an intense chronic inflammatory infiltrate, and in some areas, osteoclasts were observed surrounding the material, and no mineralization foci were found. Conclusion: It can be concluded that DDM implanted for periods of 1 or 2 months in the dorsal musculature of rats did not result in osteoinduction.


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Original Article