The effect of non-restorative treatments on the progression of artificial dentine caries lesions underneath enamel

Authors

  • Tamires Timm Maske Federal University of Pelotas
  • Camila Neunfeldt Nascimento Federal University of Pelotas
  • Françoise Hélène van de Sande Federal University of Pelotas
  • Marina Sousa Azevedo Federal University of Pelotas
  • Elenara Ferreira de Oliveira Federal University of Pelotas
  • Maximiliano Sergio Cenci Federal University of Pelotas

DOI:

https://doi.org/10.15448/1980-6523.2014.2.12753

Keywords:

Biofim, hidden caries, fluoride, chlorhexidine, demineralization

Abstract

Purpose: To develop an experimental model for hidden caries lesions and to evaluate the effect of 0.12% chlorhexidine (CHX) and 1.23% acidulated phosphate fluoride (APF) on biofilm formation and on the dentine demineralization. Methods: Dentine discs with artificial lesions were divided into four subgroups (500-100-0µm gaps or sound enamel). Enamel discs were adapted over the dentine samples and fixed in acetate matrices. Microcosm biofilms were formed on the specimens for 10 days (cariogenic condition). Specimens from subgroups (n = 9) were individually treated for 1 min by daily immersion in CHX or saline, or weekly in APF suspension. Biofilms were quantified [total microorganism (TM) and mutans streptococci (MS)]. Mineral profiles were assessed by Transverse Microradiography. Data were analyzed by ANOVA, Holm-sidak and paired t-test (p<0.05). Results: CHX decreased MT counts and increased MS counts compared to other treatments in gaps presence or absence (p<0.05). For the control, 500 µm gap caused higher TM counts (p = 0.024). No significant differences (treatment groups X subgroups) were found for the mineral profiles. Conclusion: 500 µm gap does not generate higher microbial colonization and CHX does not inhibit MS colonization of surfaces. APF and CHX showed positive trend for lesions remineralization.

Author Biography

Maximiliano Sergio Cenci, Federal University of Pelotas

Departamento Dentista Restauradora

References

Pitts NB, Davies JA. The Scottish Health Boards’ Dental Epidemiological Programme: initial surveys of 5- and 12-year-olds. Br Dent J. 1992;172: 408-13.

Macek MD, Beltran-Aguilar ED, Lockwood SA, Malvitz DM. Updated comparison of the caries susceptibility of various morphological types of. J Public Health Dent. 2003;63:174-82.

Demirci M, Tuncer S, Yuceokur AA. Prevalence of caries on individual tooth surfaces and its distribution by age and. Eur J Dent. 2010l;4:270-9.

Weerheijm KL, Gruythuysen RJ, van Amerongen WE. Prevalence of hidden caries. ASDC J Dent Child. 1992;59:408-12.

Ricketts D, Kidd E, Weerheijm K, de Soet H. Hidden caries: what is it? Does it exist? Does it matter? Int Dent J. 1997;47:259-65.

Kidd EA, Naylor MN, Wilson RF. Prevalence of clinically undetected and untreated molar occlusal dentine caries in adolescents on the Isle of Wight. Caries Res. 1992;26:397-401.

Ricketts DN, Kidd EA, Beighton D. Operative and microbiological validation of visual, radiographic and electronic diagnosis of occlusal caries in non-cavitated teeth judged to be in need of operative care. Br Dent J. 1995;179:214-20.

van de Sande FH, Azevedo MS, Lund RG, Huysmans MC, Cenci MS. An in vitro biofilm model for enamel demineralization and antimicrobial dose-response studies. Biofouling. 2011;27:1057-63.

Filoche SK, Soma KJ, Sissons CH. Caries-related plaque microcosm biofilms developed in microplates. Oral Microbiol Immunol. 2007;22:73-9.

Azevedo MS, van de Sande FH, Romano AR, Cenci MS. Microcosm biofilms originating from children with different caries experience have similar cariogenicity under successive sucrose challenges. Caries Res. 2011;45:510-7.

van Rijkom HM, Truin GJ, van ‘t Hof MA. A meta-analysis of clinical studies on the caries-inhibiting effect of chlorhexidine treatment. J Dent Res. 1996;75:790-5.

Emilson CG. Potential efficacy of chlorhexidine against mutans streptococci and human dental caries. J Dent Res. 1994 ;73:682-91.

Toledano M, Yamauti M, Osorio E, Osorio R. Zinc-inhibited MMP-mediated collagen degradation after different dentine demineralization procedures. Caries Res. 2012;46: 201-7.

Chaussain-Miller C, Fioretti F, Goldberg M, Menashi S. The role of matrix metalloproteinases (MMPs) in human caries. J Dent Res. 2006;85:22-32.

Wong L, Sissons C. A comparison of human dental plaque microcosm biofilms grown in an undefined medium and a chemically defined artificial

saliva. Arch Oral Biol. 2001;46: 477-86.

Zaura E, van Loveren C, ten Cate JM. Efficacy of fluoride toothpaste in preventing demineralization of smooth dentin surfaces and narrow grooves in situ under frequent exposures to sucrose or bananas. Caries Res. 2005;39:116-22.

Zaura E, Buijs MJ, ten Cate JM. The effects of the solubility of artificial fissures on plaque pH. J Dent Res. 2002; 81:567-71.

Lagerweij MD, Damen JJ, ten Cate JM. Effect of a fluoridated toothpaste on lesion development in plaque-filled dentine grooves: an intra-oral study. Caries Res. 1997;31:141-7.

Lagerweij MD, Damen JJ, ten Cate JM. The effect of a fluoridated toothpaste on dentinal lesions in plaque-filled grooves: an intra-oral crossover study. J Dent Res. 1996;75:1687-91.

Skold-Larsson K, Sollenius O, Petersson LG, Twetman S. Effect of topical applications of a novel chlorhexidine-thymol varnish formula on mutans streptococci and caries development in occlusal fissures of permanent molars. J Clin Dent. 2009;20:223-6.

Katz S, Park KK, Palenik CJ. In-vitro root surface caries studies. J Oral Med. 1987;42:40-8.

Hannas AR, Pereira JC, Granjeiro JM, Tjaderhane L. The role of matrix metalloproteinases in the oral environment. Acta Odontol Scand. 2007 65:1-13.

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Published

2015-06-16

Issue

Vol. 29 No. 2 (2014)

Section

Original Article

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