Comparative analysis of two fixating and two decalcifying solutions for processing of human primary teeth with inactive dentin carious lesion

Authors

  • Letícia Grando Mattuella
  • Letícia Westphalen Bento
  • Fabiana Vieira Vier-Pelisser
  • Fernando Borba Araujo
  • Anna Christina Medeiros Fossati

Abstract

The aim of this study was to analyze qualitatively the pulp tissue characteristics of maxillary anterior human primary teeth with inactive dentin carious lesion. Two buffered fixating solutions (4% paraformaldehyde and 10% formalin) and two decalcifying solutions [formic acid plus sodium citrate – Ana Morse solution and 10% ethylene-diaminetetraacetic acid(EDTA)] were evaluated. Eight teeth were assigned to 4 groups (n = 2), according to the fixating and decalcifying agents used. Group 1: 4% paraformaldehyde and Ana Morse solution; Group 2: 10% formalin and Ana Morse solution; Group 3: 4% paraformaldehyde and 10% EDTA; Group 4: 10% formalin and 10% EDTA. The teeth were fixed and thereafter decalcified until a rubber-like consistency was obtained. After histological processing, serial 6-μm-thick sections were obtained and stained with hematoxylin and eosin (H&E). Both fixating solutions provided good preservation of the pulp tissue, showing the normal tissue topography. However, the use of 10% EDTA caused tissue damage, which was evidenced by the cell aspect and the altered extracellular matrix (ECM) characteristics. Decalcification with Ana Morse solution seemed to produce better preservation of cells and ECM morphology. The association of Ana Morse solution and 10% formalin was more favorable for the pulp tissue. In conclusion, fixation with either solution yielded good tissue preservation, while decalcification with Ana Morse solution required a shorter time for specimen processing and promoted better preservation of cell components and pulpal ECM. EDTA modified the pulpal morphological properties and required a longer processing time. The combination of 10% formalin and Ana Morse solution seemed to be the most favorable for the proposed methodology.
UNITERMS: tissue fixation, decalcification, primary teeth, caries, extracellular matrix.

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Published

2007-07-03

Issue

Vol. 22 No. 56 (2007)

Section

Original Article

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