Análise em microscopia eletrônica de varredura da superfície do esmalte dentário submetido à ação de sucos de frutas cítricas
Abstract
O presente trabalho objetivou avaliar a superfície do esmalte humano submetido à ação de diferentes sucos de frutas cítricas, em microscopia eletrônica de varredura (MEV). Foram selecionados 22 dentes extraídos por razões ortodônticas, livres de trincas e/ou fraturas, sendo obtidos 44 blocos de esmalte a partir das superfícies vestibulares e linguais/palatinas destes elementos. Os blocos foram aleatoriamente divididos em 10 grupos experimentais (n = 4), submetidos ao contato por 5 minutos com: sucos diluídos (300 ml de suco + 300 ml de água) das frutas: G1 – abacaxi; G2 – caju; G3 – acerola; G4 – laranja; G5 – limão; e com sucos puros das frutas: G1’ – abacaxi; G2’ – caju; G3’ – acerola; G4’ – laranja; G5’ – limão; e um grupo controle – G6 (n = 4). O pH foi mensurado, após a preparação dos sucos. Em seguida os espécimes foram lavados em água destilada e armazenados para posterior observação em MEV. O pH registrado para os sucos foi: G1 – 3,5; G2 – 4,0; G3 – 2,8; G4 – 3,5; G5 – 1,7; G1’ – 3,6; G2’ – 3.84; G3’ – 2,84; G4’ – 3,09; G5’ – 1,64. Constatou-se a presença de áreas erosivas nos grupos experimentais que diferiam morfologicamente do esmalte registrado no grupo controle. As alterações foram mais expressivas nos grupos submetidos aos sucos de limão, acerola e abacaxi, sendo estas mais evidentes nos espécimes expostos aos sucos puros de limão e laranja e, aos sucos diluídos de acerola, abacaxi e caju. Conclui-se que, os sucos de frutas avaliados apresentaram pH ácido, demonstrando potencial erosivo, sendo este fato confirmado pelas alterações na superfície do esmalte exposto a tais líquidos.
UNITERMOS: erosão; dieta; esmalte.
SUMMARY
The objective of this work was evaluate the surface of the human dental enamel submitted to the action of different juices of citric fruits, in Sweeping Electronic microscopy (SEM). 22 human teeth were selected (premolar and third molar) extracted for orthodontic reasons, free form trines and/or fractures, being obtained 44 enamel blocks Starting from the initial surfaces and tong/Palatines of these elements. The blocks were divided in 10 experimental groups (n = 4) and submitted to the contact by 5 minutes with diluted juices (300 ml of juice + 300 ml of water) of the fruits: G1 – pineapple; G2 – cashew; G3 – acerola; G4 – orange; G5 – lemon. And a group control (G6) (n = 4). The pH was in register after the preparation of the juices. Soon after the specimens were washed in distilled water and stored for after it analyzes in SEM. The pH registered for the juice it was G1 – 3,5; G2 – 4,0; G3 – 2,8; G4 – 3,5; G5 – 1,7; G1’ – 3,6; G2’ – 3.84; G3’ – 2,84; G4’ – 3,09; G5’ – 1,64. It was evidenced presence of erosive areas in the experimental groups that had revealed morphologic aspect different from the registered in the group control. The alterations had been accented in the groups submitted to the acerola, lemon and pineapple juices, this evident in specimens displayed of lemon and orange pure juices and, acerola, pineapple and cashew diluted juices. It is unclad that, the juice as of fruits revealed pH acid, being this fact confirmed by the alternative in the surface of the exposed enamel to this liquids.
UNITERMS: erosion; diet; pH.
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