Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity

Authors

  • Carmem Dolores Vilarinho Soares Moura UFPI
  • Lorenna Bastos Lima Verde Nogueira UFPI
  • Caroline Costa Nascimento UFPI
  • Isadora Melo Vilarinho Soares UFPI
  • Janaina Cordeiro de Oliveira Castro UFPI
  • Walter Leal Moura UFPI

Keywords:

Exposure to biological agents, chlorhexidine, irreversible hydrocolloid

Abstract

Purpose: The aim of this study is to assess the reduction of microbial load that adhered to irreversible hydrocolloid impressions obtained after rinsing with 0.12% and 0.2% chlorhexidine solutions. Methods: The study consisted of 24 participants (48 dental arch impressions) equally divided into two groups: Group 1 (0.12% chlorhexidine) and Group 2 (0.2% chlorhexidine). The impressions were taken using Hydrogun ® irreversible hydrocolloid before (control) and after (experimental) a single 10 mL mouthrinse of chlorhexidine for 1 minute. Each impression was placed in a sterile beaker containing 250 ml of saline. The microbiological analysis of the storage solution was carried out using BHI Agar culture medium. The plates were incubated for 24 hours, followed by a CFU (Colony Forming Units) count. The data were analyzed using the Wilcoxon’s t test. Results: There was a reduction in the degree of contamination of the impressions after rinsing with the antimicrobial mouth rinses at both concentrations when compared with the control group. There was a statistically significant difference when the effectiveness of the two concentrations used was compared. Conclusion: Therefore, using a chlorhexidine rinse prior to obtaining irreversible hydrocolloid impressions reduces contamination and is more efficient at the 0.2% concentration.

Author Biographies

Carmem Dolores Vilarinho Soares Moura, UFPI

Aepartamento de Odontologia Restauradora

Lorenna Bastos Lima Verde Nogueira, UFPI

Student of the Masters in Dentistry, Sciences and Health Centre – Dentistry. Federal University of Piauí, Teresina - Brazil.

Caroline Costa Nascimento, UFPI

Graduate Student in Dentistry, Sciences and Health Centre – Dentistry. Federal University of Piauí, Teresina - Brazil.

Isadora Melo Vilarinho Soares, UFPI

Graduate Student in Dentistry, Sciences and Health Centre – Dentistry. Federal University of Piauí, Teresina - Brazil.

Janaina Cordeiro de Oliveira Castro, UFPI

Assistant Professor, Department of Restorative Dentistry / CCS/ Federal University of Piauí, Teresina – Brazil

Walter Leal Moura, UFPI

Associate Professor, Department of Pathology and Dental Clinic / CCS/ Federal University of Piauí, Teresina – Brazil.

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Published

2012-04-11

Issue

Vol. 27 No. 2 (2012)

Section

Original Article

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