Acute effect of the antioxidant drug U-74389G on hematocrit levels during hypoxia and reoxygenation injury in rats

Constantinos Tsompos, Constantinos Panoulis, Konstantinos Toutouzas, Aggeliki Triantafyllou, George Zografos, Apostolos Papalois


Aims: This experimental study evaluated the effect of the antioxidant drug U-74389G on hematocrit levels using a rat model of hypoxia and reoxygenation following an established protocol.

Methods: Forty rats with a mean weight of 231.875 g were employed in the study. Hematocrit levels were determined at 60 min (groups A and C) and at 120 min (groups B and D) after starting reoxygenation. Groups A and B received no drugs, whereas U-74389G was administered to rats from groups C and D. 

Results: U-74389G administration significantly increased hematocrit levels by 4.73%±2.25% (p=0.0435). Reoxygenation time increased hematocrit levels non significantly by 3.96%±2.29% (p=0.1025). U-74389G administration combined with reoxygenation time significantly increased hematocrit levels by 3.16%±1.33% (p=0.0196).

Conclusions: U-74389G administration, whether it interacted or not with reoxygenation time, significantly increased hematocrit levels in the short term in a rat model of hypoxia and reoxygenation.


hypoxia; reoxygenation; hematocrit; U-74389G; models, animal.

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Fenglin Shi, Jennifer Cavitt, Kenneth L Audus. 21-aminosteroid and 2-(aminomethyl)chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers. Free Radic Biol Med.1995;19(3):349-57.

Hori H, Kanno H. An experimental study of the protective effect of lazaroid (U-74389G) on cisplatin-induced toxicity.Nihon Jibiinkoka Gakkai Kaiho. 1999;102(1):8-18.

Schmid-Elsaesser R, Hungerhuber E, Zausinger S, Baethmann A, Reulen HJ. Neuroprotective efficacy of combination therapy with two different antioxidants in rats subjected to transient focal ischemia. Brain Res. 1999;23;816(2):471-79.

Passaquin AC, Lhote P, Rüegg UT. Calcium influx inhibition by steroids and analogs in C2C12 skeletal muscle cells.Br J Pharmacol. 1998;124(8):1751-9.

van Klaveren RJ, Pype JL, Demedts M, Nemery B. Decrease in gamma-glutamyltransferase activity in rat type II cells exposed in vitro to hyperoxia: effects of the 21-aminosteroid U-74389G. Exp Lung Res. 1997;23(4):347-59.

Schmid-Elsaesser R, Zausinger S, Hungerhaber E, Baethmann A, Reulen HJ. Neuroprotective properties of a novel antioxidant (U-101033E) with improved blood-brain barrier permeability in focal cerebral ischemia. Acta Neurochir Suppl. 1997;70:176-78.

Lehmann C, Egerer K, Georgiew A, Weber M, Grune T, Kox WJ. Inhibition of tumor necrosis factor-alpha release in rat experimental endotoxemia by treatment with the 21-aminosteroid U-74389G. Crit Care Med. 1999;27(6):1164-167.

Lehmann C, Georgiew A, Weber M, Birnbaum J, Kox WJ. Reduction in intestinal leukocyte adherence in rat experimental endotoxemia by treatment with the 21-aminosteroid U-74389G. Intensive Care Med. 2001;27(1):258-63.

Horáková L, Ondrejicková O, Bachratá K, Vajdová M. Preventive effect of several antioxidants after oxidative stress on rat brain homogenates. Gen Physiol Biophys. 2000;19(2):195-205.

Heim C, Kolasiewicz W, Sontag KH. The effects of the 21-aminosteroid U-74389G on spatial orientation in rats after a cerebral oligemic episode and iron-induced oxidative stress. J Neural Transm (Vienna). 2000;107(1):95-104.

Vlkolinský R, Stolc S. Effects of stobadine, melatonin, and other antioxidants on hypoxia/reoxygenation-induced synaptic transmission failure in rat hippocampal slices. Brain Res. 1999;850(1-2):118-26.

Durmaz R, Deliorman S, Isiksoy S, Uyar R, Erol K, Tel E. Antiproliferative properties of the lazaroids U-83836E and U-74389G on glioma cells in vitro. Pathol Oncol Res. 1999;5(3):223-8.

Kondziolka D, Somaza S, Martinez AJ, Jacobsohn J, Maitz A, Lunsford LD, Flickinger JC. Radioprotective effects of the 21-aminosteroid U-74389G for stereotactic radiosurgery. Neurosurgery. 1997;41(1):203-8.

Τsompos C, Panoulis C, Τοutouzas K, Ζografos G, Papalois A. The Acute Effect Of The Antioxidant Drug "U-74389g" On Platelet Distribution Width During Hypoxia Reoxygenation Injury In Rats. J Neurol Stroke.2015;3(6):111.

Marie I, Hervé F, Primard E, Cailleux N, Levesque H. Long-term follow-up of hypothenar hammer syndrome: a series of 47 patients. Medicine (Baltimore). 2007;86(6):334-43.

Tang YD, Rinder HM, Katz SD. Effects of recombinant human erythropoietin on antiplatelet action of aspirin and clopidogrel in healthy subjects: results of a double-blind, placebo-controlled randomized trial. Am Heart J. 2007;154(3):494.e1-7.

Nemeth N, Lesznyak T, Szokoly M, Furka I, Miko I. Allopurinol prevents erythrocyte deformability impairing but not the hematological alterations after limb ischemia-reperfusion in rats. J Invest Surg. 2006;19(1):47-56.

Cappell MS. Safety and efficacy of nasogastric intubation for gastrointestinal bleeding after myocardial infarction: an analysis of 125 patients at two tertiary cardiac referral hospitals. Dig Dis Sci. 2005;50(11):2063-70.

Götz AK, Zahler S, Stumpf P, Welsch U, Becker BF. Intracoronary formation and retention of micro aggregates of leukocytes and platelets contribute to postischemic myocardial dysfunction. Basic Res Cardiol. 2005;100(5):413-21.

Arepally A, Hofmann LV, Kim HS, Geschwind JF, Kirkwood S, Oechsle D, Perler B. Weight-based rt-PA thrombolysis protocol for acute native arterial and bypass graft occlusions. J Vasc Interv Radiol. 2002;13(1):45-50.

Ostwald P, Park SS, Toledano AY, Roth S. Adenosine receptor blockade and nitric oxide synthase inhibition in the retina: impact upon post-ischemic hyperemia and the electroretinogram. Vision Res. 1997;37(24):3453-461.

Donaldson GC, Robinson D, Allaway SL. An analysis of arterial disease mortality and BUPA health screening data in men, in relation to outdoor temperature. Clin Sci (Lond). 1997;92(3):261-68.

Cole DJ, Drummond JC, Patel PM, Reynolds LR. Hypervolemic-hemodilution during cerebral ischemia in rats: effect of diaspirin cross-linked hemoglobin (DCLHb) on neurologic outcome and infarct volume. J Neurosurg Anesthesiol. 1997;9(1):44-50.

Comes L, Mureşan A, Costin Z. Observations on isovolemic hemodilution in acute ischemic stroke. Rom J Intern Med. 1996;34(1-2):43-7.

Palmon SC, Sieber FE, Brown PR, Koehler RC, Eleff SM, Traystman RJ. Poor hemodynamic and metabolic recovery after global incomplete cerebral ischemia associated with short-term diabetes in dogs. J Cereb Blood Flow Metab.1995;15(4):673-80.

Arend SM, Bax JJ, Hermans J, van der Wall EE, Sedney MI. The short-term effect of intravenous nitroglycerin on haematocrit; an additional benefit in patients with myocardial ischaemia? Eur Heart J. 1994;15(1):114-19.

Wong JK, Pruett TL, Jones RS. Short-term effect of hepatic arterial versus portal venous reperfusion on energy levels of liver tissue. Dig Dis Sci. 1990;35(11):1397-402.

Aleksandrov PN, Shinkarenko VS, Khugaeva VK, Morozov SE. Reactions of the microcirculatory bed to transient ischemia. Fiziol Zh SSSR Im I M Sechenova.1986;72(9):1237-243.

Pozin VM, Ul'ianov MI, Khalilov EM, Pocheptsova GA, Samokhvalova IV. New hematological method for detecting long-term consequences of short-term myocardial ischemia. Biull Eksp Biol Med. 1983;96(10):119-21.

Röhnert C, Weber K, Schuster R, Lauschke G, Häcker R. Reaction of the whole body to auxiliary liver transplantation. Z Exp Chir Transplant Kunstliche Organe.1983;16(2):74-85.


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