Influência da contaminação com saliva na microinfiltração de restaurações de resina composta
Abstract
O objetivo deste estudo foi avaliar a influência da contaminação com saliva na microinfiltração marginal de restaurações adesivas. Utilizaram-se 52 incisivos bovinos, sendo confeccionadas cavidades Classe V (vestibular/lingual), com margens em dentina e esmalte. As cavidades foram condicionadas com ácido fosfórico a 35% e divididas em quatro grupos. G1 – sem contaminação, os demais grupos foram contaminados com saliva fresca por 15s, sendo submetidos a diferentes tratamentos: G2 – secas com papel absorvente; G3 – lavadas com água; G4 – recondicionadas por 10s. O sistema adesivo (Single Bond/3M ESPE) foi empregado segundo recomendações do fabricante e as cavidades restauradas com compósito (Filtek Z-250/3M ESPE). Após 24h as restaurações foram polidas e termocicladas (500 ciclos/5 e 55ºC/30s). Os dentes foram isolados, exceto as restaurações e a 1mm destas, imersos em fucsina básica a 1% (24h) e lavados em água (24h). Foram seccionados e avaliados em lupa estereoscópica, utilizando-se escores predeterminados, por dois examinadores calibrados. Os valores obtidos foram submetidos à análise estatística (Kruskal-Wallis). A infiltração em dentina foi significantemente maior que aquela observada em esmalte (p < 0,05). Nas margens em dentina observou-se redução do selamento no G2 (p < 0,05). G3 e G4 restabeleceram o selamento a níveis similares ao controle (G1). Nas margens em esmalte G1 apresentou selamento similar ao G3 e ambos exibiram melhor selamento que G2 e G4 (p < 0,05), que foram similares. Conclusões: dentro das limitações do estudo, verificou-se que a contaminação com saliva após o condicionamento ácido interfere no selamento marginal de restaurações adesivas e os tratamentos propostos apresentaram resultados diversos.
UNITERMOS: adesão; infiltração marginal; resina composta; saliva.
SUMMARY
The aim of this study was to evaluate the effect of saliva contamination on microleakage of composite restorative. Fifty-two bovine incisives were selected, Class V cavities were prepared (buccal/lingual), with margin in dentin and enamel. All cavities were 35% phosphoric acid etched and divided in four groups. G1 – there was’nt contamination; in the remaining groups, freshly collected saliva was used to contaminate for 15s and subjected to different treatments: G2 – dried with absorvent papers; G3 – washed with water; G4 – reetched for 10s. The adhesive system (Single Bond/3M ESPE) was applied according to manufacturer indications and the cavity was incrementally filled composite (Filtek Z-250/3M ESPE). The teeth were polish and thermocycled (500 cycles/5 and 55o C/30s), isolated and immersed in 1% basic fuchsin solution (24h). After washing in tap water (24h), each tooth was sectioned and the dye penetration was evaluated under magnification (40) by 2 calibrated examiners, using standard scores. Data were submitted to a nonparametric Kruskal-Wallis test with a confidence level of 95%. The dye penetration was higher in dentin than in enamel (p < 0.05). In dentin margins there was higher microleakage in G2 (p < 0.05), G3 and G4 have achieved similar values to control G1. In enamel margins, G1 exhibited similar results than G3 and both exhibited better sealing performances than G2 and G4 (p < 0.05), which were similar. Conclusions: Within the limitations of the study, it was possible to verify that saliva contamination impair the marginal quality of composite restorative and the treatments used after contamination showed different results.
UNITERMS: adhesion; composite resin; microleakage; saliva.
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