Câncer de boca: expressão imuno-histoquímica de c-erbB-2, Bcl-2 e EGFR – estudo comparativo com leucoplasia e hiperplasia inflamatória
Abstract
Anormalidades em genes que regulam a proliferação e morte celular podem provocar inúmeras doenças entre elas o carcinoma epidermóide de boca. Tem sido relatado que alterações genéticas nas células tumorais predizem a agressividade biológica dos tumores. Marcadores genéticos como c-erbB-2, Bcl-2 e EGFR são considerados indicadores promissores de prognósticos para as lesões cancerizáveis e as neoplasias. Objetivo: Avaliar a expressão imunohistoquímica das proteínas c-erbB-2, Bcl-2 e EGFR (oncoproteínas envolvidas na vias de proliferação celular). Material e Métodos: cento e cinco blocos de parafina contendo fragmentos de biopsias incisionais, sendo 54 de carcinomas epidermóides, 25 blocos de leucoplasias e 26 blocos de hiperlasias obtidos do Laboratório de Patologia de Boca da Universidade Federal de Mato Grosso do Sul (UFMS). A expressão das proteínas foi verificada através da técnica imunohistoquímica utilizando a estreptoavidina-biotina-peroxidase no Laboratório de Patologia da Universidade de Brasília (UNB). Resultados: Os resultados revelaram diferença estatisticamente significante da proteína EGFR para os carcinomas epidermóides de boca e para as demais proteínas não houve diferença estatisticamente significante entre as lesões. Conclusões: Os resultados sugerem que o EGFR pode ser utilizado como marcador em carcinoma de boca podendo contribuir para a progressão da neoplasia, porém, sendo insuficiente na predição da carcinogênese.
UNITERMOS: fatores de prognóstico; imuno-histoquímica; carcinoma epidermóide.
SUMMARY
Abnormalities in genes that regulate the proliferation and cell death can provoke many lesions as oral epidermal carcinoma. It has been related that genetic alterations in tumoral cells predict the biologic agressivity in the tumors. Genetic markers as cerbB-2, Bcl-2 and EGFR are considered indicators of prognostic to the pre-malignant lesions and neoplasias. Objective: to study in the fragments of incisional biopsy the expression of c-erbB-2, Bcl-2 and EGFR proteins. Materials and methods: 54 cases of epidermal carcinoma, 25 cases of neoplasias and 26 cases of hyperplasias obtained from Oral Pathology’s Laboratory of Federal University of Mato Grosso do Sul (UFMS). The used method was streptoavidin-biotinperoxidase in Patthology’s Laboratory of University Brasilia (UNB). Results: The results revealed positivity significant of EGFR proteins to the oral epidermal carcinoma and to the other one protein there was no statistical significant difference. Conclusions: The results suggest that EGFR can be used as a marking in oral epidermal carcinoma and can contribute to the progression of neoplasia, however, being insufficient to predict the carcinogenesis.
UNITERMS: factors of prognostic; immunohistochemical, epidermal carcinoma.
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