Antimicrobial effect of two endodontic medicaments with different exposure times, and the morphologic alterations caused to Enterococcus faecalis

Manoel Eduardo de Lima Machado, Guilherme Henrique Rosa Martins, Karine Carreira, Karina Tramontina Peixoto, Cleber Keiti Nabeshima, Ana Cristina Gales


Purpose: The aim of this study was to verify the antimicrobial effect of calcium hydroxide and iodoform on Enterococcus faecalis with different exposure times evaluating the bacterial morphologic alterations.
Methods: The antibacterial action was investigated in culture broth after zero, seven, fourteen and twenty-one days. Five mL samples were analyzed morphologically on the seventh day by transmission electron microscopy. The data of the antibacterial test were analyzed by Fisher’s exact test.
Results: The results revealed that between the seventh and fourteenth day, there was a decrease in bacterial growth with both medicaments (P = 0.098), where they were eliminated between the fourteenth and twenty-first day. Transmission electronic microscopy showed alterations in the morphologic structures.
Conclusion: It concluded that both medicaments kill Enterococcus faecalis, with an exposure time of 7 to 14 days, where no cell viability is seen after this period due to irreversible alterations in bacterial cell morphology.


Calcium hydroxide; Enterococcus faecalis; Iodoform

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e-ISSN: 1980-6523

ISSN-L: 0102-9460


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