Antimicrobial effect of two endodontic medicaments with different exposure times, and the morphologic alterations caused to Enterococcus faecalis

Authors

  • Manoel Eduardo de Lima Machado University of São Paulo
  • Guilherme Henrique Rosa Martins University of São Paulo
  • Karine Carreira Private Practice
  • Karina Tramontina Peixoto Private Practice
  • Cleber Keiti Nabeshima University of São Paulo
  • Ana Cristina Gales Federal University of São Paulo

Keywords:

Calcium hydroxide, Enterococcus faecalis, Iodoform

Abstract

Purpose: The aim of this study was to verify the antimicrobial effect of calcium hydroxide and iodoform on Enterococcus faecalis with different exposure times evaluating the bacterial morphologic alterations. Methods: The antibacterial action was investigated in culture broth after zero, seven, fourteen and twenty-one days. Five mL samples were analyzed morphologically on the seventh day by transmission electron microscopy. The data of the antibacterial test were analyzed by Fisher’s exact test. Results: The results revealed that between the seventh and fourteenth day, there was a decrease in bacterial growth with both medicaments (P = 0.098), where they were eliminated between the fourteenth and twenty-first day. Transmission electronic microscopy showed alterations in the morphologic structures. Conclusion: It concluded that both medicaments kill Enterococcus faecalis, with an exposure time of 7 to 14 days, where no cell viability is seen after this period due to irreversible alterations in bacterial cell morphology.

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Published

2011-10-16

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Section

Original Article