Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry

Authors

  • Suzana Papile Maciel Carvalho Faculdade de Odontologia de Bauru
  • Arsenio Sales-Peres Faculdade de Odontologia de Bauru-USP
  • Lucilene Arilho Ribeiro-Bicudo Hospital de Reabilitação de Anomalisa Crânio-Faciais
  • Ricardo Henrique Alves da Silva Faculdade de Odontologia de Ribeirão Preto- USP

Keywords:

Human identification, Forensic Dentistry, Forensic Medicine, DNA, molecular biology, saliva

Abstract

Purpose: This study evaluated the quality of DNA obtained from stored human saliva and its applicability to human identification. Methods: The saliva samples of 20 subjects, collected in the form of saliva in natura and from mouth swabs and stored at -20ºC, were analyzed. After 7 days, the DNA was extracted from the 40 saliva samples and subjected to PCR and electrophoresis. After 180 days, the technique was repeated with the 20 swab samples. Results: The first-stage results indicated that DNA was successfully extracted in 97.5% of reactions, 95% of saliva in natura and 100% of swab saliva samples, with no statistically significant difference between the forms of saliva. In the second phase, the result was positive for all 20 analyzed samples (100%). Subsequently, in order to analyze the quality of the DNA obtained from human saliva, the SIX3-2 gene was tested on the 20 mouth swab samples, and the PCR products were digested using the MbO1 restriction enzyme to evaluate polymorphisms in the ADRA-2 gene, with positive results for most samples. Conclusion: It was concluded that the quantity and quality of DNA from saliva and the techniques employed are adequate for forensic analysis of DNA.

Author Biographies

Suzana Papile Maciel Carvalho, Faculdade de Odontologia de Bauru

Graduação em Odontologia (FOB-USP), Especialização em Odontopediatria (HRAAC-Centrinho-USP), Mestrado em Odontologia em Saúde Coletiva (FOB-USP), Departamento Sáude Coletiva, área de Odontolgia Legal.

Arsenio Sales-Peres, Faculdade de Odontologia de Bauru-USP

Prof. Dr. Responsável pela disciplina de Odontologia Legal do departamento de Ortodontia, Odontopediatria e Odontologia em Saúde Coletica da FOB-USP, Bauru

Lucilene Arilho Ribeiro-Bicudo, Hospital de Reabilitação de Anomalisa Crânio-Faciais

Prof. Drª. Pesquisadora geneticista do Hospital de Reabilitação de Anomalias Cranio-Faciais de Bauru-USP

Ricardo Henrique Alves da Silva, Faculdade de Odontologia de Ribeirão Preto- USP

Prof. Dr. Responsável pela disciplina de Odontologia Legal no departamento de Clínica Infantil, Odontologia Preventiva e Social da Faculdade de Odontologia de Ribeirão Preto.

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Published

2010-01-27

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Original Article