Quantitative AgNORs study in ameloblastomas

Authors

  • Anna Corrêa Santos Universidade Federal de Pelotas
  • Sandra Beatriz Chaves Tarquinio Universidade Federal de Pelotas
  • Elena Riet Corrêa Rivero Universidade Federal de Santa Catarina
  • Lenita Maria Áver Araujo Universidade Federal de Pelotas
  • Cristina Iório Krause Universidade Federal de Pelotas

Keywords:

Ameloblastoma, AgNOR, cell proliferation, dentigerous cyst

Abstract

Purpose: Ameloblastomas are tumors that arise from the odontogenic epithelium, including the areas that coat the dentigerous cysts (DC). The aim of the present study was to investigate the proliferative activity present in luminal and mural areas of mural unicystic ameloblastomas (MUA), in conventional ameloblastomas (CA), and in DC, comparing them according to their biological behavior. Methods: AgNOR quantifications were performed using routine light microscopy under X100 magnification in 10 cases per type of lesion, considering 100 cells of representative areas of the tumor for each case. The AgNORs were observed as black dots within the cell nucleus. Results: MUA showed significant lower number of NORs/nucleus in the luminal proliferation area than in the area of mural proliferation and in CA (P<0.05). Conclusion: CA exhibit proliferative activity similar to the mural component of MUA, corroborating the pattern of higher aggressive clinical behavior of these tumor variants. Key words: Ameloblastoma; AgNOR; cell proliferation; dentigerous cyst

Author Biographies

Anna Corrêa Santos, Universidade Federal de Pelotas

Department of Semiology and Clinic, School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil; Especialist in Periodontology –ABO

Sandra Beatriz Chaves Tarquinio, Universidade Federal de Pelotas

Associate Professor – DDS, MS, PhD - Department of Semiology and Clinic, School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil

Elena Riet Corrêa Rivero, Universidade Federal de Santa Catarina

Associate Professor - DDS, MS, PhD Department of Pathology, Health Science Center, Federal University of Santa Catarina, Florianópolis, SC, Brazil

Lenita Maria Áver Araujo, Universidade Federal de Pelotas

Associate Professor – DDS, MS, PhD - Department of Semiology and Clinic, School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil

Cristina Iório Krause, Universidade Federal de Pelotas

DDS, Department of Semiology and Clinic, School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil

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Published

2008-11-22

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Section

Original Article