Influence of sucrose, lactose and glucose + fructose on the cariogenic potential of S. mutans: An in situ and in vitro study

Marcela Freire Borges, Aline Rogéria Freire de Castilho, Cássio Vicente Pereira


Purpose: This study evaluated the in situ effect of sucrose, lactose, and glucose+fructose on dental enamel colonization by S. mutans and its in vitro cariogenic potential.
Methods: Twelve volunteers participated in a crossover double-blind in situ study, comprising four 48-hour stages. Acrylic resin appliances with three bovine enamel blocks received a drop of experimental solution 4 times/day. The biofilm formed was inoculated in Mitis-Salivarius-Bacitracin (MSB) culture to determine the number of colonies (CFU). In the in vitro experiment, the solutions and S. mutans were added to the Brain-Heart-Infusion medium; the pH was measured after 6, 12, 24, and 48 hours; the biofilm-wet weight was determined and inoculated in MSB.
Results: There was no in situ effect of any solution on S. mutans colonization. The in vitro results showed that sucrose and lactose yielded larger number of colonies and biofilm wet-weight than glucose+fructose solution (P<0.05); sucrose samples sustained reduced pH values for a longer period of time (48 hours/pH=4.4).
Conclusion: Although there was no difference of S. mutans colonization under sucrose and lactose challenge in situ, the study vitro showed different cariogenic potential of the tested solutions.
Key words: Dental caries; microbial adhesion; Streptococcus mutans


Dental caries; microbial adhesion; Streptococcus mutans

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e-ISSN: 1980-6523

ISSN-L: 0102-9460


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