Evaluation of the aggressiveness of peripheral giant cell lesion of the oral cavity by means of a hystochemical-immunohystochemical study

João Paulo De Carli, Norberto Perri Moraes, Marcelo Macedo Crivelini, Soluete Oliveira da Silva, Maria Salete Sandini Linden, Micheline Sandini Trentin

Abstract


Purpose: To study the aggressiveness of peripheral giant cell lesion (PGCL) using hystochemical-immunohystochemical analysis.
Methods: The archives of the Dental School of Araçatuba (FOA-UNESP) and the University of Passo Fundo Dental School (FOUPF) were searched. A total of 181 PGCL cases were retrieved, and 61 met the study eligibility criteria. Firstly, the PGCL aggressiveness was graded, and the lesions were distributed into three groups based on clinical and radiographic findings. The 61 LCGPs hystopathological samples were colored by H.E. and analyzed for diagnostic confirmation. Afterwards, the cellular proliferation of 15 PGCLs cases was investigated by means of counting and calibrating of AgNORs (nucleolar organizer regions) area and evaluation of the immunoexpression of PCNA, Ki-67, and p53 markers.
Results: No difference was found for AgNOR area and PCNA expression among PGCLs of the three studied groups, and p53 was not an effective immunomarker of the analyzed lesions. There was a statistically significant difference of AgNORs number and Ki-67 expression among the three groups.
Conclusion: The AgNOR and Ki-67 results suggest that the PGCL clinical-radiographic aggressiveness can be related to its cellular proliferative activity.
Key words: Giant cell peripheric lesion; diagnosis; silver staining; immunohystochemistry

Keywords


Giant cell peripheric lesion; diagnosis; silver staining; immunohystochemistry

Full Text:

PDF


e-ISSN: 1980-6523

ISSN-L: 0102-9460

 

This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE) http://www.publicationethics.org


SJR (SCImago Journal & Country Rank) IMPACT FACTOR:

SCImago Journal & Country Rank

Licença Creative Commons

Except where otherwise noted, content of this journal is licensed under a Creative Commons Attribution 4.0 International license.

 

Editorial Policies of Brazilian Scientific Journals. Deposit availability: Blue .

 

Copyright: © 2006-2019 EDIPUCRS