Immunohistochemical expression of pericytes and myofibroblasts in the extracellular matrix of oral actinic cheilitis and squamous cell carcinoma: a comparative study

Flávia Godinho Wanderley, Carlla Silva Nunes, Maira Sá Ferreira, Ana Cristina Gonzalez, Silvia Regina Almeida Reis, Alena Ribeiro Alves Peixoto Medrado

Abstract


OBJECTIVE: The aim of the present study is to conduct a comparative analysis of oral actinic cheilitis and squamous cell carcinomas by immunohistochemical analysis.
METHODS: This cross-sectional study utilizes a convenience sample obtained from the archives of an oral pathology laboratory from a higher education institution. Tissue sections from patients diagnosed with actinic cheilitis and carcinoma were immunohistochemically analyzed using monoclonal antibodies specific for smooth muscle alpha actin (SMA) and neuron-glial antigen 2 (NG2). Individual cells that stained positive for these proteins were counted in specific fields by means of histomorphometry.
RESULTS: Subsequent statistical analysis revealed a higher number of NG2-positive when compared with SMA-positive cells in both lesion types (p = 0.005) and these cells were located adjacent to blood vessels. The differences between normal oral mucosa, severe dysplasia, and carcinoma specimens were statistically significant (p = 0.009; p = 0.006).
CONCLUSION: These data strongly suggest that increased expression of NG2- and SMA-positive cells is associated with formation of blood capillaries, highlighting the importance of angiogenesis in tumor progression.


Keywords


Carcinoma; Pericytes; Myofibroblasts

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References


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