In vitro antimicrobial effectiveness of mouthwashes against Staphylococcus aureus and Pseudomonas aeruginosa

Tereza Aparecida Delle Vedove Semenoff, Alex Semenoff Segundo, Éder Ricardo Biasoli

Abstract


Purpose: The purpose of this study was to compare the in vitro antimicrobial effectiveness of Periogard®, Cepacol®, and Plax® against Staphylococcus aureus and Pseudomonas aeruginosa.
Methods: Sixteen Petri dishes with blood agar and 16 with Mueller-Hinton agar culture media were used. Four dishes of each culture media were used as negative and positive controls. In the remaining 24 dishes, 4 wet discs were distributed in each Petri dish, as follows: 1 soaked in distilled water (control), 1 in Periogard®, 1 in Plax®, and 1 in Cepacol®. After 48h the inhibition halos of microbial growth were measured with digital calipers. Data were analyzed by ANOVA and Bonferroni test (α=0.05).
Results: Regarding P. Aeruginosa, Periogard® was significantly more effective than the other mouthwashes (P< 0.05), followed by Cepacol; Plax® and distilled water did not show inhibition of microorganism growth. Regarding S. aureus, all mouthwashes had significant inhibitory effect in the following descending order: Plax® > Periogard® > Cepacol® (P< 0.05).
Conclusion: The results suggest that the tested mouthwashes show different inhibitory potential for growth of Staphylococcus aureus and Pseudomonas aeruginosa.
Key words: Mouthwashes; Staphylococcus aureus; Pseudomonas aeruginosa; chlorhexidine; triclosan; cetylpyridinium

Keywords


Mouthwashes; Staphylococcus aureus; Pseudomonas aeruginosa; chlorhexidine; triclosan; cetylpyridinium

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e-ISSN: 1980-6523

ISSN-L: 0102-9460

 

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