Comparative analysis of microbial reduction using photodynamic therapy in bovine teeth infected with Enterococcus faecalis – an in vitro study

Janaina Guzzo Zechin, Liviu Steier, Giampiero Rossi-Fedele, Grasiela Longhi Gründling, Daniela Sá, Amanda Guwzinski, Alessandra Cesar Trindade, Matheus Albino Souza, Silvia Dias de Oliveira, Jose Antonio Poli de Figueiredo

Abstract


Objective: To evaluate the use of optic fiber and different pre-irradiation times (PIT) in phototherapy with LED for the root canal disinfection of bovine teeth infected in vitro with Enterococcus faecalis.
Methodology: The teeth were incubated for 60 days and divided into six groups according to disinfection protocol: Group 1 – distilled water; Group 2 – 1 minute PIT and no optic fiber; Group 3 – 1 minute PIT and optic fiber; Group 4 – 5 minute PIT and no optic fiber; Group 5 – 5 minute PIT and optic fiber; Group 6 – 2% sodium hypochlorite. Microbiological test (CFUs counting) and scanning electron microscopy (SEM) were performed to evaluate the effectiveness of proposed treatments.
Results: SEM analysis of canal wall, in the three thirds, revealed group 6 had the best results, whereas microbiological results revealed that group 6 had the best results. No differences in disinfection of root canals were found when optic fiber was used for photodynamic therapy. Conclusion: Photodynamic therapy should not be used alone in the disinfection of root canals, but it may be valuable as a complement for disinfection performed using different cleaning methods.


Keywords


Biofilm; Bovine teeth; Enterococcus faecalis; Photodynamic therapy

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References


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DOI: http://dx.doi.org/10.15448/1980-6523.2016.2.15365

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