Surface roughness influences Candida albicans biofilm formation on denture materials

Wander José da Silva, Letícia Machado Gonçalves, Flávia Carvalho Viu, Cristiane Maria Brasil Leal, Célia Marisa Rizatti Barbosa, Altair Antoninha Del Bel Cury

Abstract


Objective: This study aimed to evaluate the influences of surface roughness (SR) of denture base and liner materials on Candida albicans biofilm formation.
Methods: Discs were fabricated using poly (methyl methacrylate) acrylic resin and poly(ethyl methacrylate) denture liner, according to manufacturers’ instructions. Both acrylic resin and relined discs were finished or finished + polished and the SR was measured. Discs were saliva-coated, 
and C. albicans biofilms were developed on such surfaces for 48 hours. Biofilms were evaluated for cell counts, metabolic activity, and structural characteristics. Data were analyzed by one-way ANOVA and Tukey tests using a significance level of 5%.
Results: Finished acrylic resin and denture liner discs had higher SR compared to finished + polished discs (P<0.001). Finished + polished acrylic resin discs contained fewer cells compared to only finished discs (P<0.001). No metabolic activity differences were seen between biofilms formed on both groups (P>0.05). Bulky, thick and less rough biofilms were formed on only finished denture materials (P<0.05). Confocal images reveal increased presence of black spaces for biofilms developed on finished + polished discs. Conclusion: Reduced SR resulted in decreased C. albicans biofilm accumulation on both denture materials.


Keywords


Acrylic resins; Biofilms; Candida albicans

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References


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DOI: http://dx.doi.org/10.15448/1980-6523.2016.2.15324

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