Comparison of bacterial leakage between Epiphany and AH Plus sealers using single cone and Tagger’s hybrid obturation techniques

Patrícia de A. R. da Silva e Souza, Priscilla Rocha Affonso, Antônia Benedita Rodrigues Vieira, Ana Claudia Braga Amoras-Alves, Talita Tartari, Mario H. da Silva e Souza Junior


Purpose: To evaluate and compare, by means of bacterial infiltration, the quality of sealing obtained by Tagger’s hybrid (TH) and Single Cone (SC) techniques, in association with AH Plus/Gutta-percha (AH) and Epiphany/Resilon (ER).
Methods: Palatal roots of 70 maxillary molars were instrumented and divided randomly into six groups: G1, TH/AH; G2, SC/AH; G3, TH/ER; G4, SC/ER; G5, negative control; G6, positive control. The roots were sterilized and monitored for 56 days to detect bacterial leakage using Enterococcus faecalis. The data were subjected to Binomial test (α<0.05) to compare the infiltration among groups and Kruskal-Wallis test (α<0.05) to analyze the infiltration as a function of time.
Results: All positive controls leaked within 24h; none of negative controls leaked. There was no influence of time on leakage. Individual comparisons showed that there were no influences of the techniques and filling materials on quality of root canal sealing. Significant difference was found only between G1 and G4 (P=0.0229), the G1 showed a lesser and G2 the higher quantity of infiltration.
Conclusions: It could be concluded that none of the groups was able to prevent bacterial leakage and the lowest ability to prevent infiltration was obtained when applied SC/ER to filling the canal.


Bacteria; Dental materials; Endodontic; Root canal obturation; Dental leakage.

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Hommez GM, Coppens CR, De Moor RJ. Periapical health related to the quality of coronal restorations and root fillings. Int Endod J 2002;35:680-9.

Ozawa T, Taha N, Messer HH. A comparison of techniques for obturating oval-shaped root canals. Dent Mater J 2009;28:290-4.

Taşdemir T, Er K, Yildirim T, Buruk K, Celik D, Cora S, et al. Comparison of the sealing ability of three filling techniques in canals shaped with two different rotary systems: a bacterial leakage study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e129-34.

Singla M, Aggarwal V, Logani A, Shah N. Comparative evaluation of rotary

ProTaper, Profile, and conventional stepback technique on reduction in Enterococcus faecalis colony-forming units and vertical root fracture

resistance of root canals. Oral Surg Oral Med Oral Pathol Oral Radiol

Endod 2010;109:e105-10.

Fransen JN, He J, Glickman GN, Rios A, Shulman JD, Honeyman A. Comparative assessment of ActiV GP/glass ionomer sealer, Resilon/

Epiphany, and gutta-percha/AH plus obturation: a bacterial leakage study.

J Endod 2008;34:725-7.

Savariz A, González-Rodríguez MP, Ferrer-Luque CM. Long-term sealing

ability of GuttaFlow versus Ah Plus using different obturation techniques.

Med Oral Patol Oral Cir Bucal 2010;15:e936-41.

Gound TG, Sather JP, Kong TS, Makkawy HA, Marx DB. Graduating dental students’ ability to produce quality root canal fillings using singleor multiple-cone obturation techniques. J Dent Educ 2009;73:696-705.

Tagger M, Tamse A, Katz A, Korzen BH. Evaluation of the apical seal produced by a hybrid root canal filling method, combining lateral condensation and thermatic compaction. J Endod 1984;10:299-303.

Pitout E, Oberholzer TG, Blignaut E, Molepo J. Coronal leakage of teeth

root-filled with gutta-percha or Resilon root canal filling material. J Endod


Biggs SG, Knowles KI, Ibarrola JL, Pashley DH. An in vitro assessment of the sealing ability of resilon/epiphany using fluid filtration. J Endod 2006;32:759-61.

Nagas E, Uyanik MO, Sahin C, Durmaz V, Cehreli ZC. Effects of different

light-curing units and obturation techniques on the seal of the Resilon/

Epiphany system. J Endod 2008;34:1230-2.

Yilmaz Z, Tuncel B, Ozdemir HO, Serper A. Microleakage evaluation of roots filled with different obturation techniques and sealers. Oral Surg Oral

Med Oral Pathol Oral Radiol Endod 2009;108:124-8.

Onay EO, Ungor M, Orucoglu H. An in vitro evaluation of the apical sealing ability of a new resin-based root canal obturation system. J Endod 2006;32:976-8.

Pashley DH. Clinical considerations of microleakage. J Endod 1990;16:70-7.

Huybrechts B, Bud M, Bergmans L, Lambrechts P, Jacobs R. Void detection in root fillings using intraoral analogue, intraoral digital and cone beam CT images. Int Endod J 2009;42:675-85.

Lee BS, Wang CY, Fang YY, Hsieh KH, Lin CP. A novel urethane acrylatebased root canal sealer with improved degree of conversion, cytotoxicity, bond strengths, solubility, and dimensional stability. J Endod 2011;37:246-9.

Cavenago BC, Duarte MA, Ordinola-Zapata R, Marciano MA, Carpio-

Perochena AE, Bramante CM. Interfacial adaptation of an epoxy-resin

sealer and a self-etch sealer to root canal dentin using the System B or

the single cone technique. Braz Dent J 2012;23:205-11.

Paqué F, Sirtes G. Apical sealing ability of Resilon/Epiphany versus gutta-percha/AH Plus: immediate and 16-months leakage. Int Endod

J 2007;40:722-9.

Bouillaguet S, Shaw L, Barthelemy J, Krejci I, Wataha JC. Long-term sealing ability of Pulp Canal Sealer, AH-Plus, GuttaFlow and Epiphany.

Int Endod J 2008;41:219-26.

Siqueira JF, Jr., Rôças IN. Clinical implications and microbiology of bacterial

persistence after treatment procedures. J Endod 2008;34:1291-301.

Sagsen B, Er O, Kahraman Y, Orucoglu H. Evaluation of microleakage of

roots filled with different techniques with a computerized fluid filtration

technique. J Endod 2006;32:1168-70.

Veríssimo DM, do Vale MS, Monteiro AJ. Comparison of apical leakage

between canals filled with gutta-percha/AH-Plus and the Resilon/Epiphany

System, when submitted to two filling techniques. J Endod 2007;33:291-4.

Resende LM, Rached-Junior FJ, Versiani MA, Souza-Gabriel AE, Miranda CE, Silva-Sousa YT, et al. A comparative study of physicochemical properties of AH Plus, Epiphany, and Epiphany SE root canal sealers. Int Endod J 2009;42:785-93. 24. Marin-Bauza GA, Rached-Junior FJ, Souza Gabriel AE, Sousa-Neto MD, Miranda CE, Silva-Sousa YT. Physicochemical properties of methacrylate resin-based root canal sealers. J Endod 2010;36:1531-6.

Damman D, Grazziotin-Soares R, Farina AP, Cecchin D. Coronal microleakage of restorations with or without cervical barrier in root-filled teeth. Rev Odonto Ciênc 2012;27:208-12.


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