Significance of metalloproteinases in the progression of the periodontal disease

Authors

  • Flávio Roberto Guerra Seabra Potiguar University
  • Rodrigo Gadelha Vasconcelos Federal University of Rio Grande do Norte
  • Fernando José de Oliveira Nóbrega Federal University of Rio Grande do Norte
  • Éricka Janine Dantas Silveira Federal University of Rio Grande do Norte
  • Lélia Maria Guedes Queiroz Federal University of Rio Grande do Norte

DOI:

https://doi.org/10.15448/1980-6523.2014.3.11539

Keywords:

Periodontal disease, Inflammation, Matrix metalloproteinases, Immunohistochemistry.

Abstract

Objectives: The objective of this study was to evaluate the immunohistochemical expression of matrix metalloproteinases (MMPs) -1, -2 and -9 in the progression of the periodontal disease.
Materials and methods: Thirteen gingival biopsies with clinical diagnosis of gingivitis and 13 with periodontitis were obtained and processed by immunohistochemical method. Staining of MMPs was scored according to intensity, both in epithelium and in connective tissue, in absent staining (-) which was attributed the score 0; weak staining (+), score 1; and strong staining (++), score 2.
Results: MMP-1 has expressed significantly more than the others MMPs in gingivitis both in the epithelium (p=0.0008) and connective tissue (p=0.0049). In periodontitis, both MMP-1 and MMP-9 has expressed significantly in the epithelium (p<0,0001) and in the connective tissue (p=0.0002). MMP-1 and MMP-9 presented more expression in periodontitis than in gingivitis but, MMP-1 only in connective tissue (p=0,03) and MMP-9 in the epithelium (p=0.003) and in the connective tissue (p=0.04).
Conclusion: These results indicate MMP-1 have an important role in connective tissue degradation and bone loss and MMP-9, that has expressed more in periodontitis, may have some role in the
progression of gingivitis to periodontitis by acting in bone resorption.

Author Biographies

Flávio Roberto Guerra Seabra, Potiguar University

Potiguar University (UnP), Natal-RN, Brazil

Rodrigo Gadelha Vasconcelos, Federal University of Rio Grande do Norte

PhD candidate Post Graduate Program in Oral Pathology, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil

Fernando José de Oliveira Nóbrega, Federal University of Rio Grande do Norte

PhD candidate Post Graduate Program in Oral Pathology, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil

Éricka Janine Dantas Silveira, Federal University of Rio Grande do Norte

Oral Pathology Post Graduate Program, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil

Lélia Maria Guedes Queiroz, Federal University of Rio Grande do Norte

Oral Pathology Post Graduate Program, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil

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Published

2015-07-14

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