Significance of metalloproteinases in the progression of the periodontal disease

Flávio Roberto Guerra Seabra, Rodrigo Gadelha Vasconcelos, Fernando José de Oliveira Nóbrega, Éricka Janine Dantas Silveira, Lélia Maria Guedes Queiroz


Objectives: The objective of this study was to evaluate the immunohistochemical expression of matrix metalloproteinases (MMPs) -1, -2 and -9 in the progression of the periodontal disease.
Materials and methods: Thirteen gingival biopsies with clinical diagnosis of gingivitis and 13 with periodontitis were obtained and processed by immunohistochemical method. Staining of MMPs was scored according to intensity, both in epithelium and in connective tissue, in absent staining (-) which was attributed the score 0; weak staining (+), score 1; and strong staining (++), score 2.
Results: MMP-1 has expressed significantly more than the others MMPs in gingivitis both in the epithelium (p=0.0008) and connective tissue (p=0.0049). In periodontitis, both MMP-1 and MMP-9 has expressed significantly in the epithelium (p<0,0001) and in the connective tissue (p=0.0002). MMP-1 and MMP-9 presented more expression in periodontitis than in gingivitis but, MMP-1 only in connective tissue (p=0,03) and MMP-9 in the epithelium (p=0.003) and in the connective tissue (p=0.04).
Conclusion: These results indicate MMP-1 have an important role in connective tissue degradation and bone loss and MMP-9, that has expressed more in periodontitis, may have some role in the
progression of gingivitis to periodontitis by acting in bone resorption.


Periodontal disease, Inflammation, Matrix metalloproteinases, Immunohistochemistry.

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